Summary
Type: SPE
Application #: 304100
Catalog#: 730015 - SPE Cartridges, Chromabond, C18 ec, 6ml, PP, 1000mg, 30/pk
Detection: HPLC
Conditions: Column conditioning: Add 2 x 5 ml Methanol to the column and slowly aspirate. Followed by 1 x 5 ml HPLC grade water and 1 x 5 ml HPLC grade water pH 5. (Do not let run column dry.)
Sample aspiration: Aspirate the sample through the column at 10 ml/min. Vacuum dry the column for 30 seconds.
Column washing: Wash the column with 5 ml HPLC grade water pH 5.
Elution: Elute with 3 ml of Methanol.
Authors: SPE Department
Source: Macherey-Nagel